Full name: Sergey Konstantinovich Pirutin
Citizenship: Russian Federation
Nationality: Russian
Contacts:
Phone: +7-905-730-2421; +86-188-238-19051
E-mail: pirutin@yandex.ru
Education
·Candidate of Biological Sciences (Ph.D. equivalent) in Radiation Biophysics, 2002
Lomonosov Moscow State University, Faculty of Biology, Department of Biophysics, Laboratory of Radiation Physics.
Dissertation: Damaging effect of ultraviolet radiation on mouse peritoneal macrophages and modification of their photosensitivity.
·Specialist Degree in Biophysics, 1998
Lomonosov Moscow State University, Faculty of Biology, Department of Biophysics.
Diploma thesis: Study of the final stage of vesiculation on a membrane nanotube in the model system “Artificial membrane — patch-clamp pipette”.
Employment
·Shenzhen MSU-BIT University (MSU-PPI), Faculty of Biology — Researcher, Lecturer, person responsible for Biology Faculty laboratories.
·Lomonosov Moscow State University, Faculty of Biology, Department of Biophysics, Laboratory of Radiation Physics — Senior Researcher.
·Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences — Laboratory of Functional Protein Biophysics / Cell Technologies Laboratory, Senior Researcher.
·N. N. Blokhin Russian Cancer Research Center — Postgraduate studies.
·Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences — Laboratory of Bioelectrochemistry, diploma work.
Research Projects
1.Mathematical modeling of primary biophysical and biological processes.
2.Mathematical modeling of biological processes in dissipative systems.
3.Mathematical modeling of mechanisms of toxic effects of various physico-chemical factors on biological objects.
4.Mathematical modeling of ion and low-molecular compound transport across the cell membrane.
5.Studying the effects and toxicity of nanoparticles and nanomaterials in model systems using mathematical models.
6.Assessing risks of nanomaterial dissemination and impact in ecosystems and urbanized areas.
7.Building mathematical models of adaptation and resilience of biological systems to various physico-chemical factors.
8.Developing and testing artificial model ecosystems to assess risks of various physico-chemical factors.
9.Biophysical and mathematical approaches to environmental state assessment and ecological monitoring.
10.Damaging effect of ultraviolet radiation on mouse peritoneal macrophages and modification of their photosensitivity.
11.Permeability of cell membranes after UV irradiation and repair of membranes after such damaging exposure; mathematical modeling of these processes.
12.Studying changes in conductance of a bilayer lipid membrane induced by an electric field.
13.Studying the effect of external-beam gamma therapy on luminol-dependent and lucigenin-dependent chemiluminescence of human phagocytes isolated from peripheral blood in oncological diseases; searching for mathematical models correlating with experimental data.
14.Studying the effects of heavy metals and their nanoparticles on primary photosynthetic processes.
Research Methods and Skills
Molecular dynamics; mathematical and computer modeling; numerical methods in biophysics; mathematical and computational data processing; spectral data analysis; Raman spectroscopy; single-cell microfluorimetry; chemiluminescence analysis; UV-visible absorption and fluorescence spectroscopy; light, fluorescence, and confocal microscopy; scanning electron microscopy; patch-clamp technique; microinjection; immunohistochemistry; cell culture maintenance; PCR; flow cytometry.
Teaching Experience
Shenzhen MSU-BIT University (MSBU), Shenzhen
·Lectures on the physical foundations of Raman spectroscopy; practical classes in Raman spectroscopy; mathematical and statistical principles for processing Raman spectra.
·Physics seminars, applying differential and integral calculus to solve physics problems.
·Introductory lectures on practical biophysical methods; use of mathematical modeling in biophysics; practical classes in biophysics, mathematical and statistical processing of spectra and datasets; the course includes tasks on mathematical and computer modeling of enzymatic biochemical processes based on the Michaelis–Menten equation.
·Supervision of postgraduate students (currently supervised a Ph.D. student who successfully completed postgraduate studies at MSBU). Research topic: study of nanoparticle effects on biological objects in model systems; investigation of cellular effects of nanoparticles using an interdisciplinary biophysical and mathematical approach to assess risks at different levels of biological organization.
·Supervision of bachelor’s and master’s theses.
Lomonosov Moscow State University, Moscow
·Lectures for graduate students on cellular biophysics and mathematical modeling of cellular processes.
·Practical classes in single-cell microfluorimetry and chemiluminescence analysis; mathematical and statistical principles of data processing.
Management Experience
·Laboratory manager for the Biology Faculty laboratories at MSBU.
·Process engineer in food production: process management and quality control.
Selected Publications:
- Пирутин С.К., Шанк М.А., Цзя Ш., Конюхов И.В., Тодоренко Д.А., Червицов Р.Н., Фурсова П.В., Кабашникова Л.Ф., Плюснина Т.Ю., Хрущев С.С., Ризниченко Г.Ю., Рубин А.Б. Комплексный анализ воздействия ионов меди на первичные процессы фотосинтеза Scenedesmus quadricauda по результатам измерений флуоресценции хлорофилла a в суспензии и на одиночных клетках // Компьютерные исследования и моделирование. – 2025. – Т. 17, №2. – C.293-322.
- Pirutin S., Chaikovskii D., Shank M., Chivarzin M., Jia S., Yusipovich A., Suvorov O., Zhao Y., Bezryadnov D., Rubin A. Investigation of cell damage induced by silver nanoparticles in a model cell system // Pharmaceutics. – 2025. – Vol.17, No.4. – P. 398. DOI: https://doi.org/10.3390/pharmaceutics17040398.
- Parshina E.Yu, Liu W., Yusipovich A.I., Gvozdev D.A., He Y., Pirutin S.K., Klimanova E.A., Maksimov E.G., Maksimov G.V. Spectral and conformational characteristics of phycocyanin associated with changes of medium pH // Photosynthesis Research, Springer Nature (Switzerland). 15 January. 2024. https://doi.org/10.1007/s11120-023-01068-0.
- Pirutin S.K., Jia S., Yusipovich A.I., Shank M.A., Parshina E.Y., Rubin A.B. Vibrational spectroscopy as a tool for bioanalytical and biomonitoring studies // International Journal of Molecular Sciences. – 2023. – Vol.24, No.8. – P.6947. DOI: https://doi.org/10.3390/ijms24086947.
- Yusipovich Alexander I., Allakhverdiev Elvin S., Parshina E.Yu, Pirutin Sergey K., Silicheva Margarita A., Rodnenkov Oleg V., Martynyuk Tamila V., Maksimov Georgy V. Hemoglobin Heme Conformation in Patients with Different Oxygen Saturation Values // Journal of Biomedical Photonics & Engineering, Samara National Research University (Samara), 2022. Vol. 8, № 4, с. 040505-1-040505-6 DOI: 10.18287/JBPE22.08.040505.
- A. I. Yusipovich, E. Yu. Parshina, A. A. Baizhumanov, S. K. Pirutin, A. D. Ivanovc, V. L. Minaevd, G. G. Levin and G. V. Maksimov. Use of a Laser Interference Microscope for Estimating Fluctuations and the Equivalent Elastic Constant of Cell Membranes. // ISSN 0020-4412, Instruments and Experimental Techniques, 2021, Vol. 64, No. 6, pp. 877–885.
- Druzhko Anna B., Pirutin Sergey K. Investigation of spectral and kinetic properties of polymer films based on someanalogs of bacteriorhodopsin. // European Biophysics Journal, издательство Springer Verlag (Germany), 2019. том 48, № 8, с. 749-756 DOI: 10.1007/s00249-019-01401-3.
- Kazakova T.A., Yusipovich A.I., Pirutin S.K., Maksimov G.V. // Changes in the Frequency of Rhythmic Excitation of Retzius Cells during Thermal Stimulation of Leech Skin Bulletin of Experimental Biology and Medicine. 2020. Voll.168, P.378-380
-Druzhko Anna B., Pirutin Sergey K. // Investigation of spectral and kinetic properties of polymer films based on someanalogs of bacteriorhodopsin. European Biophysics Journal. 2019. Voll. 48. № 8. P. 749-756
- Pirutin S.K., Efremova M.V., Yusipovich A.I., Turovetskii V.B., Maksimov G.V., Druzhko A.B., Mazhuga A.G. Visualization and Cytotoxicity of Fluorescence-Labeled Dimeric Magnetite-Gold Nanoparticles Conjugated with Prostate-Specific Membrane Antigen in Mouse Macrophages Bulletin of Experimental Biology and Medicine, Springer Nature (Switzerland), 2019. Voll. 166. № 3. P. 386-389
-Pirutin S., Turovetskii V., Kudryashov Yu Influence of pH on the UV sensitivity of peritoneal macrophage plasma membrane Biophysics, Maik Nauka/Interperiodica Publishing 2010. Voll. 55, № 1, с. 148-150
- Pirutin S.K., Turovetskii V.B., Kudriashov Iu B.Alteration of UV-sensitivity of mice peritoneal macrophage plasma membranes by calcium ions Radiation biology. Radioecology, 2011. Voll. 51. № 4. P. 419-424
-Korchemskaya E, Burykin N, de Lera A, Alvarez R, Pirutin S, Druzhko A Study of 14-fluorine bacteriorhodopsin gelatin films for dynamic holography recording. Photochemistry & Photobiology, 2005, 81: 920-923
